Cytokine-induced neutrophil chemoattractant mediates neutrophilic alveolitis in rats: association with nuclear factor kappa B activation.

摘要: Cytokine-induced neutrophil chemoattractant (CINC) is a rat cytokine with structural and functional homology to human interleukin-8 (IL-8) melanoma growth-stimulatory activity (MGSA/gro). We investigated the relationship between CINC production of chemotactic for neutrophils by alveolar macrophages after in vitro vivo treatment endotoxin. After endotoxin, bioactivity produced increased time- dose-dependent manner. This increase was closely associated levels steady-state mRNA. About 50% blocked neutralizing concentrations anti-CINC antibodies. then evaluated role development neutrophilic alveolitis rats, which results from single intraperitoneal injection In this model, peak numbers are recovered lung lavage fluid 24 h endotoxin injection. Steady-state mRNA peaked 2 Many cytokines whose transcription induced during sepsis, including IL-8 MGSA/gro, thought be transcriptionally regulated nuclear factor kappa B (NF-kappa B). The gene contains binding site promoter region NF-kB. Therefore, we sought determine whether NF-kappa NK-kappa motif extracts cells exposure using gel mobility shift assays. Increased detected 2.5 endotoxin.(ABSTRACT TRUNCATED AT 250 WORDS)