Cellular distribution of nuclear factor κB binding activity in rat liver

摘要: The cellular localization of nuclear factor kappa B (NF-kappa B) binding activity in rat liver has been investigated using electrophoretic mobility shift assay on extracts highly purified hepatocytes and Kupffer cells obtained from perfused vivo with collagenase. Constitutive NF-kappa was demonstrated control cells, this not apparently influenced by injection lipopolysaccharide (LPS) into rats 24 h before perfusion. In contrast, little present animals, although there detectable inactive, inhibitor-bound, the cytoplasm. However, increased LPS-treated animals after vitro culture hepatocytes. Thus appears to be inducible both vitro. These findings support a role for hepatocyte gene regulation which may important modulation hepatic acute phase response.