Expression of Castanea crenata Allene Oxide Synthase in Arabidopsis Improves the Defense to Phytophthora cinnamomi

摘要: ABSTRACT Allene oxide synthase (AOS) is a key enzyme of the jasmonic acid (JA) signaling pathway. The AOS gene was previously found to be upregulated in an Asian chestnut species resistant infection by oomycete Phytophthora cinnamomi (Castanea crenata), while lower expression values were detected susceptible European sativa). Here, we report genetic and functional characterization C. crenata (CcAOS) upon its heterologous ecotype Arabidopsis thaliana, which contains single gene. It that plants expressing CcAOS delay pathogen progression exhibit more vigorous growth presence. They also show upregulation salicylic acid-related genes. As native species, localized plastids, as revealed confocal imaging CcAOS-eGFP fusion protein transgenic roots. This observation confirmed transient Nicotiana benthamiana leaf epidermal cells. To further confirm specific role defense mechanism against pathogen, performed crosses between infertile knockout mutant line. normal growth, remain but are significantly tolerant than wild type plants. Together, our results indicate important player plant responses varieties may valuable tool mitigate biotic stress responses. One-sentence summary Heterologous Castanea allene thaliana improves response cinnamomi.