Immunocytochemical localization of group III metabotropic glutamate receptors in the hippocampus with subtype-specific antibodies

摘要: The metabotropic glutamate receptors (mGluRs) mGluR4 and mGluR7 have been postulated to serve as presynaptic autoreceptors in the hippocampal formation. cellular synaptic localization of these proteins hippocampus, however, is not known. Polyclonal antibodies that specifically react with mGluR4a or were produced used for immunocytochemical receptor proteins. Immunoblot analysis mGluRs expressed stably transfected baby hamster kidney cells suggests each reacts appropriate mGluR subtype but other mGluRs. Both recognized native rat brain molecular weights similar bands mGluR-transfected cell lines. distribution immunoreactivity was fairly uniform all regions. Immunoreactivity variable different regions closely paralleled previously reported mRNA. Immunocytochemistry light electron microscopy (EM) revealed immunoreactivities are widely differentially distributed throughout Staining directed against intense bodies dendrites pyramidal cells, granule scattered interneurons. Analysis at EM level postsynaptic asymmetrical (presumably glutamatergic) synapses both symmetrical synapses. largely this These data consistent a role hippocampus suggest may functions.