Molecular Characterization and Developing a Point-of-Need Molecular Test for Diagnosis of Bovine Papillomavirus (BPV) Type 1 in Cattle from Egypt

摘要: Bovine papillomatosis is a viral disease of cattle causing cutaneous warts. A diagnosis this infection very mandatory for combating the resulting economic losses. Given limited data available about bovine papillomavirus (BPV) in Egypt, present study involved molecular type-1 (BPV-1), -2, -4, -5, and -10 presenting warts on head neck from New Valley Province, Egypt. The phylogenetic analysis detected types BPV was also performed, followed by developing point-of-need assay rapid identification identified types. In regard, total 308 private farms Egypt were clinically examined, which 13 animals presented due to suspected infection. symptomatic treated surgically, biopsies skin lesions collected BPV-1, using polymerase chain reaction (PCR). presence BPV-1 DNA confirmed 11 samples (84.6%), while BPV-2, not detected. Sequencing PCR products suggested Egyptian virus closely related found India. An isothermal nucleic acid amplification test (NAAT) with labeled primers specific L1 gene sequence, based recombinase (RPA), combination lateral flow strip detection RPA products, developed tested. demonstrated diagnostic utility comparable PCR-based testing. Taken together, provides interesting occurrence reveals genetic relatedness buffalo addition, simple, low-cost combined validated suggests necessity future investigations circulating strains among assess their better understand epidemiological pattern disease.