Less cytotoxicity to combination therapy of 5-fluorouracil and cisplatin than 5-fluorouracil alone in human colon cancer cell lines

作者: Xiu-Xu Chen , Mao-De Lai , Yong-Liang Zhang , Qiong Huang

DOI: 10.3748/WJG.V8.I5.841

关键词:

摘要: AIM: Our previous studies showed increased sensitivity to 5-FU in colon cancer cell lines with microsatellite instability, and considered that mutations of TGFβ-R II, IGF IIR, RIZ gene might enhance the potentials growth proliferation, which 5-FU. Here we compared distribution cycle P53 status between two human different Because mechanistic differences exist CDDP, also analyzed efficacy CDDP combination therapy on lines. METHODS: We these by MTT assay. Distribution under treatment 5-FU, alone or both was Flow Cytometry, expression detected immunocytochemical staining. RESULTS: SW480 cells were more sensitive than LoVo at concentrations above 16 μmol/L (Ratio absorption is 0.64 0.79 μmol/L, respectively; P < 0.01). Efficacy conversely lower single-therapy + 0.53, 0.54, 0.72, 0.78, negative whereas positive expression. induced G1-phase arrest lines, but peaked 24 h earlier cells, 48 for an apparent hypodiploid DNA. However, contrary, inducing S-phase arrest, peaking 36 earlier. Both hypodipliod nuclei after treatment. Percentage dominated alternatively lines. CONCLUSION: These results suggest instability are resistant CDDP. Combination shows fewer efficacies single-therapy, although it can render a arrest. may be involved shift S-phase, inessentially.

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