Thermophilic Anaerobic Biodegradation of [14C]Lignin, [14C]Cellulose, and [14C]Lignocellulose Preparations

摘要: Thermophilic (55 degrees C) anaerobic enrichment cultures were incubated with [C-lignin]lignocellulose, [C-polysaccharide]lignocellulose, and kraft [C]lignin prepared from slash pine, Pinus elliottii, C-labeled preparations of synthetic lignin purified cellulose. Significant but low percentages (2 to 4%) natural pine recovered as labeled methane carbon dioxide during 60-day incubations, whereas much greater (13 23%) gaseous end products. Percentages label lignin-labeled substrates dissolved degradation products approximately equal High-pressure liquid chromatographic analyses CuO oxidation sound degraded indicated that no substantial chemical modifications the remaining polymer, such demethoxylation dearomatization, occurred biodegradation. The polysaccharide components lignocellulose cellulose relatively rapidly mineralized dioxide; 31 37% polysaccharides 56 63% An additional 10 20% was Overall, these results indicate elevated temperatures can greatly enhance rates lignified low-molecular-weight aromatic compounds.