Responsiveness of human monocyte-derived dendritic cells to thimerosal and mercury derivatives

摘要: Abstract Several cases of skin sensitization have been reported following the application thimerosal, which is composed ethyl mercury and thiosalicylic acid (TSA). However, few in vitro studies carried out on human dendritic cells (DCs) play an essential role initiation allergic contact dermatitis. The aim present study was to identify effect thimerosal other compounds DCs. To address this purpose, DCs derived from monocytes (mono-DCs) were used. Data show that derivatives induced DC activation, as monitored by CD86 HLA-DR overexpression associated with secretion tumor necrosis factor α interleukin 8, similarly lipopolysaccharide sensitizers, 1-chloro-2,4-dinitrobenzene (DNCB) nickel sulfate, used positive controls. In contrast, TSA, non-mercury part well dichloronitrobenzene, a DNCB negative control, irritant, sodium dodecyl had no effect. Moreover, oxidative stress, ROS induction depolarization mitochondrial membrane potential, compounds, DNCB, comparison hydrogen peroxide, control. thiol oxidation mono-DC activation confirmed pre-treatment N -acetyl- l -cysteine strongly decreased chemical-induced overexpression. These data are agreement several clinical observations high relevance patch-test reactions prove mono-DCs useful tools for determining allergenic potency chemicals.