Spread of the emerging equine-like G3P[8] DS-1-like genetic backbone rotavirus strain in Brazil and identification of potential genetic variants.

摘要: In 2013, the equine-like G3P[8] DS-1-like rotavirus (RVA) strain emerged worldwide. 2016, this was reported in northern Brazil. The aims of study were to conduct a retrospective genetic investigation identify possible entry these atypical strains Brazil and describe their distribution across representative area country. From 2013 2017, total 4226 faecal samples screened for RVA by ELISA, PAGE, RT-PCR sequencing. represented 20.9 % (167/800) all RVA-positive samples, further subdivided as G3P[8], (11.0 %; 88/800) Wa-like (9.9 79/800). Six selected whole-genome investigation, confirming backbone I2-R2-C2-M2-A2-N2-T2-E2-H2. During 2013–2014, predominant no detected. Equine-like first identified Parana March/2015, suggesting that entered through Southern region. rapidly spread under surveillance displayed marked potential replace strains. Brazilian clustered with contemporary detected worldwide, but exhibited distinct NSP2 genotype (N2) compared previously Amazon (N1). Two NSP4 E2 lineages also identified. Taken together, data suggest different variants might have been introduced into country at time points, co-circulated period 2015–2017. global emergence strains, predominantly countries using Rotarix vaccine, raises question whether vaccines may be inducing selective pressures on zoonotic