A dual cancer-specific recombinant adenovirus suppresses the growth of liver cancer cells in vivo and in vitro.

摘要: Oncolytic virus therapy is emerging as important means in cancer treatment. In a previous study, we constructed dual cancer-specific antitumor recombinant adenovirus, designating it Ad-apoptin-hTERTp-E1a (Ad-VT). This study aimed to investigate the anticancer potential of adenovirus (Ad-VT) liver cancer. Crystal Violet staining and CCK-8 assays were used analyse inhibitory effect on human hepatoma cell line QGY-7703 SMMC-7721. Ad-VT had significant tumour killing SMMC-7721 cells that was both dose time dependent. Ad-VT-induced apoptosis detected using Hoechst, Annexin V, JC-1 staining, well western blotting. Recombinant strong apoptosis-inducing cells, killed mainly through mitochondrial apoptotic pathway. invasion cell-scratch Transwell assays. could significantly inhibit over short period time. The pGL4.51 plasmid transfect construct stably expressing luciferase (QGY-7703-LUC). inhibition vivo subsequently confirmed by establishing tumour-bearing nude mouse model. effectively growth prolong survival mice. has characteristics tumour-specific replication specific killing, promote their apoptosis.