Antitumor effects of a dual cancer-specific oncolytic adenovirus on colorectal cancer in vitro and in vivo.
作者： GUOHUA YANG , XIANGWEI MENG , LILI SUN , NINGNING HU , SHUANG JIANG
关键词: Oncolytic virus 、 Oncolytic adenovirus 、 Immunology 、 Genetic enhancement 、 Cancer research 、 In vivo 、 Cancer 、 Cytotoxic T cell 、 Biology 、 Apoptosis 、 Oncogene
摘要: The efficacy and specificity of treatment are major challenges for cancer gene therapy. Oncolytic virotherapy is an attractive drug delivery platform In the present study, dual-specific antitumor oncolytic adenovirus, Ad-Apoptin-hTERT-E1a, was used to infect SW1116 human colorectal carcinoma (CRC) cell lines CT26 mouse-CRC-cell bearing BALB/c mouse models testing effects in vitro vivo. assays revealed that infection with Ad-Apoptin-hTERT-E1a induced a significant cytotoxic effect on CRC line, SW1116; however, normal GES, only slightly inhibited by recombinant adenovirus. Acridine orange ethidium bromide staining annexin V assay indicated cells resulted induction apoptosis. Furthermore, western blotting flow cytometry decrease mitochondrial membrane potential (MMP), release cytochrome c activation caspase 3, 6 7 Ad-Apoptin-hTERT-E1a-infected cells. animal models, shown significantly inhibit tumor growth extend survival times animals. Therefore, experimental results has application
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