Simultaneous imaging of intracellular [Ca2+] and pH in single MDCK and glomerular epithelial cells.

摘要: The interrelationships between changes in intracellular calcium concentration ([Ca2+]i) and pH Madin-Darby canine kidney cells glomerular epithelial exposed to various stimuli were analyzed simultaneously using a new design of fluorescence video microscope. Cells double labeled with indo 1 SNARF dyes excited at 350 540 nm. Images four emission wavelengths captured 405, 475, 575, 640 nm 30 frames/s for the two ratio dyes. sensitivity 6.5 8.0 was unchanged by [Ca2+]i. maps used correct pH-dependent calculation local cell calcium. NH4Cl loading produced expected alkalinization concurrent rise When removed became acidic, second [Ca2+]i recorded. Both from stores since they persisted absence extracellular findings demonstrate need correction recordings.