Crosstalk between cytosolic pH and intracellular calcium in human lymphocytes:

摘要: Stimulation of lymphocytes by specific antigens is followed the activation different signal transduction mechanisms, such as alterations in cytoplasmic levels Ca 2 ! ,H and variations membrane potential. To study interrelationships among these parameters, changes pHi were measured with fluorescent probes BCECF Fura-2 freshly isolated blood human lymphocytes. Moreover, potential qualitative recorded dye bis- oxonol. In a bicarbonate-free medium, cell alkalinization NH 4 Cl slightly decreased intracellular concentration ((Ca ) i due to efflux from cell. contrast, an elevation induced 4-AP increased (Ca , either presence or absence external . The increase -free medium likely be release thapsigargin caffeine- independent stores. Both plasma depolarisation, although kinetics. ionosphere ionomycin pHi, also depolarisation. Together, observations demonstrate lack correlation between magnitude