Serratia marcescens hemolysin (ShlA) binds artificial membranes and forms pores in a receptor-independent manner

摘要: Both the inactive and active conformations of hemolysin/cytolysin Serratia marcescens (ShlA) binds membranes erythrocytes, but only ShlA is able to form pores. unable lyse prokaryotic membranes. To determine receptors binding pore-forming domains cytolysin on eukaryotic membranes, artificial large unilamellar vesicles (LUVs) various membrane compositions were examined. In current study, it shown that significant pore formation lysis was achieved with binary phosphatidylcholine/phosphatidylserine (PS) liposomes. No proteinaceous receptor needed for either or by ShlA. Membrane integration activity enhanced addition phosphatidylethanolamine. Phosphatidylserine negatively charged at physiologic pH almost absent in Hence, insertion are highly dependent phosphatidylserine, which targets toxic cell without any need a receptor. This may explain why found be resistant against previous study.