Phylogenetic analysis of long-chain hydrocarbon-degrading bacteria and evaluation of their hydrocarbon-degradation by the 2,6-DCPIP assay.
作者： Kenzo Kubota , Daisuke Koma , Yoshiki Matsumiya , Seon-Yong Chung , Motoki Kubo
DOI: 10.1007/S10532-008-9179-1
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摘要: Thirty-six bacteria that degraded long-chain hydrocarbons were isolated from natural environments using (waste car engine oil, base oil or the c-alkane fraction of oil) as sole carbon and energy source. A phylogenetic tree isolates constructed their 16S rDNA sequences revealed divided into six genera plus one family (Acinetobacter, Rhodococcus, Gordonia, Pseudomonas, Ralstonia, Bacillus Alcaligenaceae, respectively). Furthermore, most (27 36) classified Acinetobacter, Rhodococcus Gordonia. The hydrocarbon-degradation similarity in each strain was confirmed by 2,6-dichlorophenol indophenol (2,6-DCPIP) assay. Isolates belonging to genus Acinetobacter normal alkanes (n-alkanes) but did not degrade short-chain n-alkanes cyclic (c-alkanes), while Gordonia both c-alkanes.
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