Computer-controlled freezing of rainbow trout Oncorhynchus mykiss (Walbaum) spermatozoa for routine programmes

作者: I. Babiak , L. Fraser , S. Dobosz , K. Goryczko , H. Kuzminski

DOI: 10.1046/J.1365-2109.1999.00381.X

关键词:

摘要: The effects of extender composition and freezing rate on cryopreservation efficiency refrigerated spermatozoa rainbow trout Oncorhynchus mykiss (Walbaum) were evaluated in order to test the suitability a computer-controlled ultrafreezer cryopreserve milt samples obtained field conditions stored for several hours. A very highly significant first-order interaction between type was found. Six eight experimental variants did not differ significantly, resulting, after fertilization eggs with cryopreserved sperm, range 62.3–74.8% eyed embryos. This procedure effective at 1 °C 2 days.

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