Optimisation in chromatography

摘要: Abstract Plate theory is applied to the separation of eluted peaks in chromatography. It shown that peak elution volumes VA and VB for two solutes A B can be used define a factor S [S = (VB - VA)/(VA + VB)] which simply related chromatographic resolution Rs; 2Rs/N± where N number theoretical plates column. use relative volatility α optimisation performance lead inconsistencies when column dead volume VO not much smaller than avoids these more straightforward particularly since it does require measurement VO. Application both approaches made pH aromatic acids water-methanol on octadecyl silica.