Multi-variate ranking function for optimizing separations

摘要: Abstract Systematic methods development requires that the quality of a chromatogram be quantified so improvement in given separation can proceed an orderly fashion. The estimate chromatographic quality, though arguably subjective, needs to expressed as function standard parameters, such resolution and retention time. Functions rely on single parameter often fail adequately evaluate complex chromatograms, because inherent trade-offs between We have devised formula incorporates both time along with third factor, peak distribution. This produced results agreed subjective criteria for chromatograms containing up fifteen components. Not only rankings but also relative visual interpretation. differences allow optimization process path greatest slope (improvement). Our provides ordinal cardinal ranking used establish historical norms.