Folding and persistence times of intramolecular G-quadruplexes transiently embedded in a DNA duplex

摘要: G-quadruplex (G4) DNA structures have emerged as important regulatory elements during metabolic transactions. While many in vitro studies focused on the kinetics of G4 formation within single-strands, are found vivo double-stranded regions, where their is challenged by complementary strand. Since energy hybridization Watson-Crick dominates folding, this competition should play a critical role persistence. To address this, we designed single-molecule assay allowing to measure folding and persistence times presence We quantified both unfolding rates biologically relevant sequences, such cMYC cKIT oncogene promoters, human telomeres an avian replication origin. confirmed that G4s much more stable tested origin promoters than telomere repeats. In addition, characterized how dynamics was affected ligands showed rate time increased. Our opens new perspectives for measurement mimicking fork, which understand gene regulation at mechanistic level.