A major part of platelet-derived growth factor purified from human platelets is a heterodimer of one A and one B chain
作者： A Hammacher , U Hellman , A Johnsson , A Ostman , K Gunnarsson
DOI: 10.1016/S0021-9258(18)37619-1
关键词:
摘要: Platelet-derived growth factor, PDGF, purified from human platelets is a disulfide-bonded dimer consisting of two homologous polypeptide chains denoted A and B; it has not been known whether heterodimer or mixture homodimers. We present here evidence that major part PDGF structure. highly homogeneous, 31-kDa was in the presence protease inhibitors shown to contain both by means immunoprecipitations with peptide antisera specific for B chains, respectively. The susceptibility mild acid treatment its chromatographic behavior reversed-phase high performance liquid chromatography immobilized metal ion affinity chromatography, as compared chain homodimers, consistent Analysis according our routine, large scale procedure revealed have In addition, homodimers were also found. With demonstration occurs heterodimer, all three dimeric forms identified. following nomenclature distinguish various suggested: PDGF-AA, homodimer chains; PDGF-AB, heterodimer; PDGF-BB, any form chains.
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