Agmatine protects against intracerebroventricular streptozotocin-induced water maze memory deficit, hippocampal apoptosis and Akt/GSK3β signaling disruption

摘要: Centrally administered streptozotocin (STZ), is known to cause Alzheimer׳s like memory deterioration. It mainly affects insulin signaling pathways such as PI3/Akt and GSK-3β which are involved in cell survival. Previous studies indicate that STZ increases the ratio of Bax/Bcl-2 thereby induces caspase-3 activation apoptosis. Agmatine, a polyamine derived from l-arginine decarboxylation, recently shown exert some neuroprotective effects. This study aimed assess if agmatine reverses STZ-induced deficits, hippocampal Akt/GSK-3β disruption activation. Adult male Sprague-Dawely rats weighing 200-250 g were used. The canules implanted bilaterally into lateral ventricles. was on days 1 3 (3 mg/kg) treatment (40 or 80 started day 4 continued an every other manner till 14. animal׳s learning capability assessed 15-18 using Morris water maze. After complement behavioral hippocampi isolated amounts cleaved (the landmark apoptosis), ratio, total phosphorylated forms Akt analyzed by western blot. results showed but not 40 mg/kg reversed deterioration induced STZ. Western blot analysis revealed prompted elevation caspase-3; disrupted hippocampus. Agmatine prevented apoptosis impairment disclosed averts only also disruption.