RAPID CLONAL PROPAGATION OF CHINESE MEDICINAL HERBS BY TISSUE CULTURE

作者: T. G. Gau , D. D. Chang , Y. C. Chern , C. C. Chen , F. T. Yeh

DOI: 10.1007/978-94-011-1779-1_53

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摘要: Adventitious buds could be regenerated from different source organs of medicinal herbs, for examples, terminal or lateral bud, bulbil, immature leaf blade, petiole, stem segment and root tip. Differences in the ability regeneration were observed among plant organs. For instance, Pinellia ternata, bulbils showed highest regenerative followed by blade petiole. Whereas, shoot tips Anoectochilus fomosanus, Rehumannia glutinosa Platycodon gradiflorum superior to other terms differentiation into adventitious buds. Ability vegetative reproduction maintained alternative solid-and liquid-medium culture. Rooting was easy significant when 1/2 strength MS medium with 3% sucrose 1 mg/1 NAA (for P. ternata grandiflorum) without any added growth regulator R. A. formosanus). Plants vitro culture morphologically similar field-grown plants. Thus we conclude that tissue is a very useful system mass propagaation above-mentioned herbs.

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