An improved method of in vitro propagation ofDioscorea bulbifera
作者： Clare Forsyth , J. Van Staden
DOI: 10.1007/BF02318924
关键词: Improved method 、 Diosgenin 、 Tissue culture 、 New crop 、 Dioscorea bulbifera 、 Kinetin 、 Shoot 、 In vitro 、 Botany 、 Biology
摘要: Nodal stem segments ofDioscorea bulbifera were induced to form plantlets in vitro. Rooted obtained on Murashige-Skoog [14] revised medium supplemented initially with 5 mg 1−1 kinetin and subsequently l−1 indole-butyric acid. By increasing the concentration from 10 l−1, number of shoots forming per node was increased five eight. When substituted 6-benzylaminopurine at only 1 nine formed each node. Each shoot could be excised a new crop multiple shoots. successfully transferred vivo conditions.
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