Spatiotemporal Uncoupling of MicroRNA-Mediated Translational Repression and Target RNA Degradation Controls MicroRNP Recycling in Mammalian Cells.

摘要: MicroRNA (miRNA)-mediated repression controls expression of more than half protein-coding genes in metazoan animals. Translation is associated with target mRNA degradation initiated by decapping and deadenylation the repressed mRNAs. Earlier evidence suggests endoplasmic reticulum (ER) as site where microRNPs (miRNPs) interact their targets before translation sets in, but subcellular location subsequent miRNA-repressed messages largely unidentified. Here, we explore distribution essential components machineries miRNA-targeted We have noted that interaction mRNAs AGO2 protein on ER precedes relocalization to multivesicular bodies (MVBs). The subsequently get deadenylated, lose AGO2, become decapped. Blocking maturation endosomes late endosome MVBs targeting endosomal HRS uncouples miRNA-mediated from RNA degradation. also targeted intracellular parasite Leishmania donovani, which curtails level infected cells prevent uncoupling mRNA-AGO2 interaction, preventing translationally messages, thus stops recycling miRNPs preengaged repression.