LncRNA KCNQ1OT1 sponges miR-34c-5p to promote osteosarcoma growth via ALDOA enhanced aerobic glycolysis.
作者： Yifei Shen , Jingwen Xu , Xiaohui Pan , Yunkun Zhang , Yiping Weng
DOI: 10.1038/S41419-020-2485-1
关键词:
摘要: Metabolic switch from oxidative phosphorylation to aerobic glycolysis, which is also called the Warburg effect, a hallmark of osteosarcoma (OS) and leads enhancement cell chemoresistance, growth, metastasis, invasion. Emerging evidence indicates that long non-coding RNA (lncRNA) plays crucial role in effect cancer cells. Here, we report lncRNA KCNQ1OT1 was upregulated OS. Meanwhile, functional experiments demonstrated facilitated proliferation suppressed apoptosis OS In addition, contributed by stimulating aldolase A (ALDOA) expression. Furthermore, using bioinformatics analysis, luciferase reporter, immunoprecipitation, pull-down assay, identified functions as competing endogenous (ceRNA) sponging miR-34c-5p, inhibited ALDOA expression directly targeting its 3'UTR. Taken together, these data key glucose metabolism reprogramming Targeting KCNQ1OT1/miR-34c-5p/ALDOA axis may be potential therapeutic target treatment.
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