EGFR antisense oligonucleotides encapsulated with nanoparticles decrease EGFR, MAPK1 and STAT5 expression in a human colon cancer cell line.
作者： Ahmad Gholamhoseinian Najar , Roghiyeh Pashaei-Asl , Yadollah Omidi , Safar Farajnia , Ali Reza Nourazarian
DOI: 10.7314/APJCP.2013.14.1.495
关键词:
摘要: Epidermal growth factor receptor (EGFR) is over-expressed in several human cancers. This would suggest that inhibition of EGFR a reasonable approach for cancer treatment. In this study we investigated blocking and its effects on the mediated signaling such as MAPK STATb HT29 cells. For aim used FITC-labeled antisense oligonucleotides encapsulated with PAMAM nanoparticles to inhibit expression. Cellular uptake was by fluorescence microscopy flow cytometry analysis. The effect expression cells examined real time PCR Western blots, which showed decreased level mRNA protein. addition, results confirmed had an effective role reduction dependent downstream genes. conclusion, down regulated EGFR-mediated
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researchgate.net 参考文章(30)
Kelly M. Quesnelle, Amanda L. Boehm, Jennifer R. Grandis, STAT‐mediated EGFR signaling in cancer Journal of Cellular Biochemistry. ,vol. 102, pp. 311- 319 ,(2007) , 10.1002/JCB.21475
F Ciardiello, G Magliulo, D S Salomon, N Normanno, V Damiano, C Bianco, A R Bianco, M P Selvam, G Tortora, M Grassi, E de Angelis, J Mendelsohn, Growth inhibition of human colon carcinoma cells by combinations of anti-epidermal growth factor-related growth factor antisense oligonucleotides. Clinical Cancer Research. ,vol. 2, pp. 601- 609 ,(1996)
Daniel J. Hicklin, Lee M. Ellis, Harlan W. Waksal, Andrea T. Hooper, Marie C. Prewett, Rajiv Bassi, Enhanced Antitumor Activity of Anti-epidermal Growth Factor Receptor Monoclonal Antibody IMC-C225 in Combination with Irinotecan (CPT-11) against Human Colorectal Tumor Xenografts Clinical Cancer Research. ,vol. 8, pp. 994- 1003 ,(2002)
J. R. Testa, A. Bellacosa, AKT plays a central role in tumorigenesis. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 98, pp. 10983- 10985 ,(2001) , 10.1073/PNAS.211430998
M. Francoual, M.-C. Etienne-Grimaldi, J.-L. Formento, D. Benchimol, A. Bourgeon, M. Chazal, C. Letoublon, T. André, N. Gilly, J.-R. Delpero, P. Lasser, J.-P. Spano, G. Milano, EGFR in colorectal cancer: more than a simple receptor Annals of Oncology. ,vol. 17, pp. 962- 967 ,(2006) , 10.1093/ANNONC/MDL037
R. T. Greenlee, T. Murray, S. Bolden, P. A. Wingo, Cancer statistics, 2000. CA: A Cancer Journal for Clinicians. ,vol. 50, pp. 7- 33 ,(2000) , 10.3322/CANJCLIN.50.1.7
Pei Wang, Xin-Han Zhao, Zhi-Yu Wang, Min Meng, Xu Li, Qian Ning, Generation 4 polyamidoamine dendrimers is a novel candidate of nano-carrier for gene delivery agents in breast cancer treatment Cancer Letters. ,vol. 298, pp. 34- 49 ,(2010) , 10.1016/J.CANLET.2010.06.001
Jing Yuan Fang, Bruce C Richardson, The MAPK signalling pathways and colorectal cancer. Lancet Oncology. ,vol. 6, pp. 322- 327 ,(2005) , 10.1016/S1470-2045(05)70168-6
Raj Kumar, Sowmyalakshmi Srinivasan, Pallab Pahari, Jürgen Rohr, Chendil Damodaran, Activating stress-activated protein kinase-mediated cell death and inhibiting epidermal growth factor receptor signaling: a promising therapeutic strategy for prostate cancer. Molecular Cancer Therapeutics. ,vol. 9, pp. 2488- 2496 ,(2010) , 10.1158/1535-7163.MCT-10-0180
Rebecca Siegal, KD Miller, Ahmddin Jemal, Cancer statistics, 2012 CA: A Cancer Journal for Clinicians. ,vol. 62, pp. 10- 29 ,(2012) , 10.3322/CAAC.20138