Alterations of epithelial stem cell marker patterns in human diabetic corneas and effects of c-met gene therapy

摘要: Purpose We have previously identified specific epithelial proteins with altered expression in human diabetic central corneas. Decreased hepatocyte growth factor receptor (c-met) and increased proteinases were functionally implicated the changes of these diabetes. The present study examined whether limbal stem cell marker patterns corneas c-met gene overexpression could normalize patterns. Methods Cryostat sections 28 ex vivo 26 organ-cultured autopsy normal by immunohistochemistry using antibodies to putative markers including ATP-binding cassette sub-family G member 2 (ABCG2), N-cadherin, ΔNp63α, tenascin-C, laminin γ3 chain, keratins (K) K15, K17, K19, β(1) integrin, vimentin, frizzled 7, fibronectin. Organ-cultured studied upon transduction adenovirus harboring gene. Results Immunostaining for ABCG2, was significantly decreased cell-harboring basal epithelium either intensity or number positive cells. Basement membrane components, fibronectin (but not tenascin-C) also showed a significant reduction limbus. c-Met transduction, which normalizes wound healing, accompanied staining α(3)β(1) compared vector-transduced Conclusions data suggest that compartment is long-term Gene therapy, such as overexpression, be able restore function corneal