Single-Cell Profiling of Epigenetic Modifiers Identifies PRDM14 as an Inducer of Cell Fate in the Mammalian Embryo
作者： Adam Burton , Julius Muller , Shengjiang Tu , Pablo Padilla-Longoria , Ernesto Guccione
DOI: 10.1016/J.CELREP.2013.09.044
关键词:
摘要: Cell plasticity or potency is necessary for the formation of multiple cell types. The mechanisms underlying this are largely unknown. Preimplantation mouse embryos undergo drastic changes in cellular potency, starting with totipotent zygote through to pluripotent inner mass (ICM) and differentiated trophectoderm blastocyst. Here, we set out identify functionally characterize chromatin modifiers that define transitions fate embryo. Using a quantitative microfluidics approach single cells, show developmental marked by distinctive combinatorial profiles epigenetic modifiers. Pluripotent cells ICM distinct from their counterparts. We PRDM14 heterogeneously expressed 4-cell-stage embryos. Forced expression at 2-cell stage leads increased H3R26me2 can induce fate. Our results shed light on networks govern identity vivo.
core.ac.uk 
sci-hub.se 参考文章(44)
Morito Sakaue, Hiroshi Ohta, Yuichi Kumaki, Masaaki Oda, Yuko Sakaide, Chisa Matsuoka, Akiko Yamagiwa, Hitoshi Niwa, Teruhiko Wakayama, Masaki Okano, DNA Methylation Is Dispensable for the Growth and Survival of the Extraembryonic Lineages Current Biology. ,vol. 20, pp. 1452- 1457 ,(2010) , 10.1016/J.CUB.2010.06.050
Erna Magnúsdóttir, Sabine Dietmann, Kazuhiro Murakami, Ufuk Günesdogan, Fuchou Tang, Siqin Bao, Evangelia Diamanti, Kaiqin Lao, Berthold Gottgens, M Azim Surani, None, A tripartite transcription factor network regulates primordial germ cell specification in mice Nature Cell Biology. ,vol. 15, pp. 905- 915 ,(2013) , 10.1038/NCB2798
D. Chen, Regulation of transcription by a protein methyltransferase. Science. ,vol. 284, pp. 2174- 2177 ,(1999) , 10.1126/SCIENCE.284.5423.2174
Ryutaro Hirasawa, Hiroyuki Sasaki, Dynamic transition of Dnmt3b expression in mouse pre- and early post-implantation embryos. Gene Expression Patterns. ,vol. 9, pp. 27- 30 ,(2009) , 10.1016/J.GEP.2008.09.002
Eran Meshorer, Dhananjay Yellajoshula, Eric George, Peter J. Scambler, David T. Brown, Tom Misteli, Hyperdynamic Plasticity of Chromatin Proteins in Pluripotent Embryonic Stem Cells Developmental Cell. ,vol. 10, pp. 105- 116 ,(2006) , 10.1016/J.DEVCEL.2005.10.017
Jennifer Nichols, Branko Zevnik, Konstantinos Anastassiadis, Hitoshi Niwa, Daniela Klewe-Nebenius, Ian Chambers, Hans Schöler, Austin Smith, Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4 Cell. ,vol. 95, pp. 379- 391 ,(1998) , 10.1016/S0092-8674(00)81769-9
Guoji Guo, Mikael Huss, Guo Qing Tong, Chaoyang Wang, Li Li Sun, Neil D. Clarke, Paul Robson, Resolution of Cell Fate Decisions Revealed by Single-Cell Gene Expression Analysis from Zygote to Blastocyst Developmental Cell. ,vol. 18, pp. 675- 685 ,(2010) , 10.1016/J.DEVCEL.2010.02.012
Toshio Hamatani, Mark G. Carter, Alexei A. Sharov, Minoru S.H. Ko, Dynamics of Global Gene Expression Changes during Mouse Preimplantation Development Developmental Cell. ,vol. 6, pp. 117- 131 ,(2004) , 10.1016/S1534-5807(03)00373-3
Brian B. Tuch, Edith Heard, Kaiqin Lao, M. Azim Surani, Fuchou Tang, Catalin Barbacioru, Ellen Nordman, Siqin Bao, Caroline Lee, Xiaohui Wang, Deterministic and Stochastic Allele Specific Gene Expression in Single Mouse Blastomeres PLoS ONE. ,vol. 6, pp. e21208- ,(2011) , 10.1371/JOURNAL.PONE.0021208
Sarayu Ratnam, Carmen Mertineit, Feng Ding, Carina Y. Howell, Hugh J. Clarke, Timothy H. Bestor, J.Richard Chaillet, Jacquetta M. Trasler, Dynamics of Dnmt1 methyltransferase expression and intracellular localization during oogenesis and preimplantation development. Developmental Biology. ,vol. 245, pp. 304- 314 ,(2002) , 10.1006/DBIO.2002.0628