The Trace and Subgrouping of Lymphocyte Activation by Dynamic Fluorescence Intensity and Polarization Measurements

摘要: Cell activation involves conformational changes of cytosolic enzymes, and/or their regulatory proteins, as well intracellular matrix re-organization. In this work, these were monitored by dynamic measurements fluorescence polarization in single cells incubated with or without phytohaemagglutinin (PHA), using the Cellscan mark S (CS-S) cytometer. This instrument and procedure used proved to be a powerful tool for distinguishing subpopulations cells. Grouping staining rates (the time rate change intensity) yielded three major subgroups. For each subgroup, depolarization (FDP) induced incubation PHA was measured. The kinetics subgroups indicate that FDP is contributed lowest rate. approximately 1.5 times greater than bulk population. It believed analysis kinetic probing might yield an important more sensitive method functional marking response characteristics.