Antitumor activity of magainin analogues against human lung cancer cell lines.
作者： Adi F. Gazdar , Bruce E. Johnson , Hao Chia Chen , Yoshinobu Ohsaki
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摘要: Magainin 1 and magainin 2, originally isolated from African clawed frog Xenopus laevis skin, inhibit the growth of bacteria fungi. Synthetic A (MAG A) G G) are more potent against protozoa. In order to determine antitumor activity these analogues, we have tested two analogues six small cell lung cancer (SCLC) lines NCI-H82, NCI-H526, NCI-H678, NCI-H735, NCI-H841, NCI-H889, which were known differ by than 10-fold in their sensitivity different chemotherapeutic agents, four normal human fibroblast lines. Semiautomated 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays SCLC revealed average concentrations producing 50% inhibition (IC50) values 2.6 microM (range, 0.49-9.30 microM) for cisplatin, 2.5 0.39-6.00 etoposide, 138.8 nM 55.0-450.0 nM) doxorubicin. The IC50 MAG was 8.64 6.23-11.7 that 8.82 4.44-12.5 Despite a difference standard differs less 3-fold. 21.1 12.7-25.6 29.2 21.3-34.8 G. Combined exposure concentration or plus etoposide cisplatin decreased percentage surviving cells 29.0% 26.1-31.7%). had an additive effect when used with agents. These data suggest vitro
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