Antibacterial Activities of Peptides Designed as Hybrids of Antimicrobial Peptides
作者： Hyung Keun Kim , Dong Gun Lee , Yoonkyung Park , Hee Nam Kim , Bo Hwa Choi
关键词:
摘要: Hybrid peptides (HP-MA, HP-ME), each of 20 residues and incorporating 2–9 Helicobacter pylori ribosomal protein L1 (HP) 1–12 magainin 2 melittin, were designed. The antibiotic activities these evaluated using bacterial, tumor human erythrocyte cells. HP-MA had a stronger antibacterial activity against Gram-positive bacteria Gram-negative than HP (2-20) 2, HP-ME was similar to melittin. None the hybrids anti-tumor or hemolytic activity. These further investigated an artificial liposomal vesicle 1,6-diphenyl-1,3,5-hexatriene as membrane probe, confirmed have activities. effect is probably caused by their ability damage bacterial plasma membrane. Additional circular dichroism spectra suggested that α-helical structure plays important role in but property less connected with enhanced
springer.com
koreascience.or.kr参考文章(8)
David Andreu, Josep Ubach, Anita Boman, Birgitta Wåhlin, David Wade, R.B. Merrifield, Hans G. Boman, Shortened cecropin A-melittin hybrids. Significant size reduction retains potent antibiotic activity. FEBS Letters. ,vol. 296, pp. 190- 194 ,(1992) , 10.1016/0014-5793(92)80377-S
Hao-Chia Chen, Judith H. Brown, John L. Morell, C.M. Huang, Synthetic magainin analogues with improved antimicrobial activity FEBS Letters. ,vol. 236, pp. 462- 466 ,(1988) , 10.1016/0014-5793(88)80077-2
M. T. Tosteson, S. J. Holmes, M. Razin, D. C. Tosteson, Melittin lysis of red cells. The Journal of Membrane Biology. ,vol. 87, pp. 35- 44 ,(1985) , 10.1007/BF01870697
H.G. Boman, D. Wade, I.A. Boman, B. Wåhlin, R.B. Merrifield, Antibacterial and antimalarial properties of peptides that are cecropin-melittin hybrids FEBS Letters. ,vol. 259, pp. 103- 106 ,(1989) , 10.1016/0014-5793(89)81505-4
B Merrifield, Solid phase synthesis Science. ,vol. 232, pp. 341- 347 ,(1986) , 10.1126/SCIENCE.3961484
M. Zasloff, Magainins, a class of antimicrobial peptides from Xenopus skin: isolation, characterization of two active forms, and partial cDNA sequence of a precursor Proceedings of the National Academy of Sciences of the United States of America. ,vol. 84, pp. 5449- 5453 ,(1987) , 10.1073/PNAS.84.15.5449
Robert C. MacDonald, Ruby I. MacDonald, Bert Ph.M. Menco, Keizo Takeshita, Nanda K. Subbarao, Lan-rong Hu, Small-volume extrusion apparatus for preparation of large, unilamellar vesicles Biochimica et Biophysica Acta. ,vol. 1061, pp. 297- 303 ,(1991) , 10.1016/0005-2736(91)90295-J
Adi F. Gazdar, Bruce E. Johnson, Hao Chia Chen, Yoshinobu Ohsaki, Antitumor activity of magainin analogues against human lung cancer cell lines. Cancer Research. ,vol. 52, pp. 3534- 3538 ,(1992)