Placental uptake and metabolism as determinants of pregnancy vitamin D status

摘要: Abstract Pregnancy 25-hydroxyvitamin D (25(OH)D) concentrations are associated with maternal and fetal health outcomes, but the underlying mechanisms have not been elucidated. Using physiological human placental perfusion approaches intact villous explants we demonstrate a role for placenta in regulating relationships between 25(OH)D physiology. Here, active uptake of 25(OH)D3 by endocytosis metabolism into 24,25-dihydroxyvitamin D3 1,25-dihydroxyvitamin [1,25(OH)2D3], subsequent release these metabolites both circulations. Active transport synthesis 1,25(OH)2D3 that supply is dependent on function rather than solely availability 25(OH)D3. We exposure induces rapid effects transcriptome proteome. These map to multiple pathways central thereby development, independent vitamin transfer, including transcriptional activation inflammatory responses. Our data suggest epigenetic landscape helps dictate response treatment. This first quantitative study demonstrating transfer placenta; widespread itself. show complex synergistic interplay placenta, inform possible interventions optimise better support growth adaptations pregnancy. Significance Statement Maternal linked development postnatal health, adverse events pregnancy, does necessarily predict supply. If, passive diffusion, determine quantity type reaching fetus this becomes regulated process. Furthermore, specific proteome patterns relevant transfer. landscape. Therefore, alone sufficient outcome: critical ensuring receives optimal during intrauterine life.