Precursors with glial fibrillary acidic protein promoter activity transiently generate GABA interneurons in the postnatal cerebellum.

摘要: Neural stem or progenitor cells (NSC/NPCs) able to generate the different neuron and glial cell types of cerebellum have been isolated in vitro, but their identity location intact are unclear. Here, we use inducible Cre recombination GFAPCreERT2 mice irreversibly activate reporter gene expression at P2 (postnatal day 2), P5, P12 with GFAP (glial fibrillary acidic protein) promoter activity analyze fate genetically tagged vivo. We show that P2-P5 β-galactosidase enhanced green fluorescent proteins genes least 30% basket stellate GABAergic interneurons molecular layer (ML) they lose neurogenic potential by P12, after which only glia. Tagged cerebellar white matter (WM) were initially GFAP/S100β+ expressed NSC/NPCs LeX, Musashi1, Sox2 One week tagging, reporter+ WM upregulated neuronal markers Mash1, Pax2, Gad-67. These Pax2+ progenitors migrated throughout cortex, populating ML leaving P18. data suggest a pool located large fraction for within first postnatal 12 days development. This restricted critical period implies powerful inhibitory factors may restrict vivo later stages Stem Cells 2009;27:1152–1163