Chromogranins, widespread in endocrine and nervous tissue, bind Ca2+.

摘要: The proteinaceous components of the secretory vesicle contents isolated from bovine adrenal medulla bind Ca2+ (number binding sites, 152 ± 52 nmol per mg protein; dissociation constant, 54 8 μM (n = 5)). SDS-polyacrylamide gel electrophoresis and 45Ca2+binding proteins following their separation blotting on nitrocellulose revealed that binds to chromogranins. Moreover, it was shown chromogranins, like other known Ca2+-binding proteins, can be specifically stained with a cationic carbocyanine dye. function chromogranins described here, in conjunction recent findings concerning transport across chromaffin membranes widespread distribution many different endocrine nerve cells, points general importance these metabolism ofCa2+.