Chromogranin A, the major catecholamine storage vesicle soluble protein. Multiple size forms, subcellular storage, and regional distribution in chromaffin and nervous tissue elucidated by radioimmunoassay.

摘要: Chromogranin A (CgA), the major catecholamine storage vesicle (CSV) soluble protein, may index exocytotic sympathoadrenal secretion. To explore CgA in adrenergic tissues, we developed a radioimmunoassay for bovine CgA. Within adrenal medulla CSV, several minor chromogranins had similar amino acid compositions and peptide maps to that of also showed parallel, partial cross-reactivity radioimmunoassay. immunoreactivity represented 7 +/- 1% total cell protein was localized representing 46 2% CSV protein. In brain, there 1000-fold less than medulla, with widespread regional distribution (maximal neocortex) an unusual subcellular cytosol), both which differ from reported distribution. Brain chromogranin lower Stokes radius Sympathetic nerve serum 6,000-fold 30,000-fold medulla. The results suggest "family" chromogranins, structurally immunoligically. Adrenal brain concentration, localization, molecular size. Finally, provide useful tool studies intact organisms.