In vitro analysis of the interactions of recombinant IL-2 with regenerating lymphoid and myeloid cells after allogeneic marrow transplantation.

摘要: Although administration of rIL-2 post-T depleted allogeneic bone marrow transplantation (TD-BMT) offers the prospect augmenting immune reconstitution and thereby reducing risks infection relapse, it has been unclear what direct or indirect effects this agent would have on regenerating myeloid system. We find that addition 200 IU to patient lymphocytes obtained within 6 wk TD-BMT results in a substantial (2 3 log) increase INF-gamma secretion production TNF. Cytokines present supernatants from IL-2-stimulated two contrasting cells normal donors recipients. They prime granulocytes for enhanced oxidative metabolism as measured by ability generate chemiluminescence response FMLP, whereas IL-2 added directly neutrophils no effect. However, these IL-2-induced cytokines also act inhibit progenitor growth reduce granulocyte macrophage (GM) colony formation mean 53%. Preincubation with anti-IFN-gamma antibody partially abrogates both enhancement suppression CFU-GM. Addition recipient produces inhibition, leading 25% reduction GM-colony growth. This effect is not due interaction between IL-2, because completely abrogated removal CD8 Leu-7+ marrow. suppressive vitro are particular concern after BMT, potential promote function, reconstitution, anti-leukemic activity justify further consideration therapy setting.