STUDIES ON RAT LIVER CATALASE. I. COMBINED IMMUNOCHEMICAL AND ENZYMATIC DETERMINATION OF CATALASE IN LIVER CELL FRACTIONS.

摘要: Enzymatic activity measurements give incomplete information on the distribution of an enzyme among cell components. By combining enzymatic measurement with immunochemical assay, existence inactive forms enzyme, or inhibitors activators, can frequently be detected. Use a precipitin procedure has added advantage that protein is isolated as it assayed, and incorporation labeled substrates followed. The catalase in rat liver fractions terms been reported by Von Euler Heller (I), Ludewig Chanutin (2)) Greenfield Price (3), Thomson Klipfel (4), de Duve et al. (5), Fourcade Rosenberg (6). approach employed bovine (7-g)) mouse (lo), (11). However, studies have not yet applied to fractions. In previous application method liver, Higashi, Yagi, Hirai (11) antiserum against human red blood which precipitated some extent. present work purified preparation obtained antigen. Large scale preparations mitochondria were used starting material for purification, since was desired avoid possible heterogeneity due multiple within cell. For version (12) selected, permitted complete recovery fairly pure components also yielded lysosome-rich fraction. Separate made “rough surfaced” “smooth microsomes nuclei. these ascertained both methods. Values two methods good agreement except microsomes, result 2 3 times one.