Inhibitory effects of metabolite I of erdosteine on the generation of nitric oxide and peroxynitrite chemiluminescence by human neutrophils.

作者: Monica Dal Sasso , Maria Culici , Tiziana Bianchi , Elena Fonti , Pier Carlo Braga

DOI: 10.1159/000077445

关键词:

摘要: Polymorphonuclear neutrophils (PMNs) can generate superoxide anions and nitric oxide (NO), which is not only an important mediator of various cellular activities, but also react with to produce peroxynitrite (ONOO–). Peroxynitrite a potent potentially toxic oxidant that damages types biomolecules. It preferentially mediates the oxidation thiolic groups in protein non-protein molecules, thus altering their functions. The aim this study was examine whether, addition its ability reduce respiratory bursts human PMNs, SH metabolite I (Met I) erdosteine, interfere NO NO-derived production, extending antioxidant activity. This done by means luminol amplified chemiluminescence (LACL), has been widely used detect production reactive species (ROS) PMNs under conditions. At 5 10 µg/ml, Met significantly reduced LACL after fMLP PMA stimulation. When L-Arg added reaction medium, as donor, increased up 67% 132%, once again µg/ml I. In cell-free system, use linsidomine (SIN-1) makes it possible investigate behavior induced release, concentrations ranging from 1.25 µg/ml. Our findings indicate I, molecule group, reacts ROS, peroxynitrite, both scavenging interest for strategy protecting against damage radical pulmonary cell environment, they induce phlogogenic loop, suggests adding exogenous thiols may be useful antagonizing effects molecules on endogenous thiols.

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