Identification and validation of QTLs for salt tolerance during vegetative growth in tomato by selective genotyping.

摘要: The purpose of this study was to identify quantitative trait loci (QTLs) for salt tolerance (ST) during vegetative growth (VG) in tomato by distributional extreme analysis and compare them with the QTLs previously identified trait. A BC1 population (N = 792) a cross between moderately salt-sensitive Lycopersicon esculentum Mill. breeding line (NC84173, maternal recurrent parent) salt-tolerant L. pimpinellifolium (Jusl.) accession (LA722) evaluated ST solution cultures containing 700 mM NaCl + 70 CaCl2 (electrical conductivity, EC 64 dS/m phiw approximately -35.2 bars). Thirty-seven plants (4.7% total) that exhibited highest were selected (referred as population), grown maturity greenhouse pots self-pollinated produce BC1S1 progeny seeds. 37 families their average performance compared parental before selection. realized heritability 0.50 obtained population. subjected restriction fragment length polymorphism (RFLP) using 115 markers, marker allele frequencies determined. Allele same markers also determined an unselected 119) cross. trait-based (TBA), which measures differences populations, used marker-linked QTLs. Five genomic regions detected on chromosomes 1, 3, 5, 6, 11 bearing significant ST. Except QTL chromosome all had positive alleles contributed from tolerant parent LA722. Of five QTLs, three (those 5) another study, thus validated here. Only one major our previous not This high level conformity results two studies indicates genuine nature potential usefulness marker-assisted selection (MAS). few most or comparable These should be useful development lines via MAS.