Identification of common dermatophytes (Trichophyton, Microsporum, Epidermophyton) using polymerase chain reactions

摘要: Polymerase chain reaction (PCR) fingerprinting detected DNA polymorphisms among frequently isolated species and strains of the genera Trichophyton, Microsporum Epidermophyton. The patterns generated by this DNA-based method permitted to be identified. conventional methods identify dermatophytes rely on expression characteristic morphological features, as well several physiological properties. Identification is often delayed or problematic because isolates may slow form conidia produce atypical microscopic structures colony appearances. Using non-specific primers such (AC)10, (GTG)5, M13 core sequence AP3, PCR profiles were for 17 species. Intraspecies variables also observed four six varieties T. mentagrophytes, whereas no detectable variability was found within three tonsurans. Comparing species-specific fingerprints clinical with those type strains, could identified their fingerprints, even if they not accepted phenotypic characteristics.