Maspin Expression Profile in Human Prostate Cancer (CaP) and in Vitro Induction of Maspin Expression by Androgen Ablation

摘要: Purpose: Expression of tumor suppressor gene, MASPIN , is associated with inhibition cell invasion and metastasis. Loss or decreased expression Maspin found frequently in breast prostate cancer cells. The objective this study to investigate specimens explore the mechanisms hormonal regulation tumors. Experimental Design: Immunohistochemical staining was performed on surgical whole-mounted specimens. scored individual Correlation clinicopathological features analyzed for statistical significance. Androgen ablation-induced by promoter luciferase reporter assay quantitative reverse transcription-PCR analysis endogenous LNCaP cells vitro animal model. Results: Comprehensive evaluation profile multiple foci from whole mounted patients revealed absence a significant fraction (63%). However, significantly higher (92%) treated neoadjuvant androgen ablation therapy before radical prostatectomy. cultured androgen-depleted medium show induction activity assay. In addition, increased after castration derived tumors nude mice. Conclusions: absent primary cancers. Up-regulation response strongly suggests physiological role growth and/or apoptosis during ablation.