Biomarker discovery in urine by proteomics.

摘要: A hypothesis was formed that it would be possible to isolate an adequate amount of protein from a patient, having normal renal function, identify biological markers particular disease state using variety proteomics techniques. To support this hypothesis, three samples urine were collected volunteer: first when healthy, later experiencing acute inflammation due pilonidal abcess, and again still after successful recovery the condition. The these processed by solid-phase extraction concentrate desalt endogenous proteins peptides. peptides analyzed in different experiments: (1) traditional two-dimensional gel electrophoresis followed proteolysis mass spectrometric identification various spots, (2) whole mixture one-dimensional packed capillary liquid chromatography tandem spectrometry, (3) spectrometry. In all cases, set identified representing putative biomarkers. Each then found have been previously linked scientific literature inflammation. One phase reactant particular, orosomucoid, readily observed experiments dramatically increase abundance, thereby supporting hypothesis.