Chapter 7 – Applications of Coherent Raman Spectroscopy: Bacterial Spore Detection via Optimized Pulse Configuration

摘要: Publisher Summary The anxiety caused by anthrax scares that turned out to be false alarms was further aggravated the length of time required analyze powderlike substance. need for real-time detection methods, local and remote, became apparent. It triggered a large-scale search suitable techniques in different fields expertise, including biology, chemistry, nanoengineering, laser spectroscopy. most prominent biological methods are polymerase chain reaction (PCR) immunoassays. PCR employs primers separate organic-specific nucleic acid sequences polymerases amplify segment until it is detectable. chapter explores possibilities using coherent Raman spectroscopy biohazards. By exciting vibrational coherence on more than one transition simultaneously, we aiming robust definitive means obtain molecule-specific signal use species identification. In particular, concentrates dipicolinic (DPA), known marker molecule bacterial spores. considers time- frequency-resolved ways adapt those this particular application.