Depletion of carboxypeptidase E, a regulated secretory pathway sorting receptor, causes misrouting and constitutive secretion of proinsulin and proenkephalin, but not chromogranin A.
作者： Emmanuel Normant , Y. Peng Loh
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摘要: Previous studies have shown that the prohormone POMC is sorted to regulated secretory pathway (RSP), at trans-Golgi network, by binding of a conformation-dependent sorting signal receptor, identified as membrane-bound carboxypeptidase E (CPE) (Cool et al., 1997, Cell, 88:73–83). In this study, role CPE receptor for other RSP proteins contain signals (proinsulin, proenkephalin, and chromogranin A) was investigated in neuroendocrine cells (Neuro-2a) stably expressing antisense RNA. Whereas these were depleted greater than 85%, electron microscopy showed they dense core granules identical wild-type Neuro-2a cells, indicating not essential granulogenesis. Secretion immunocytochemical that, endogenous proenkephalin transfected proinsulin/insulin localized punctate released via RSP. However, CPE-depleted...
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