Subunits of the H+-ATPase of Escherichia coli. Overproduction of an eight-subunit F1F0-ATPase following induction of a lambda-transducing phage carrying the unc operon.
作者： D.L. Foster , M.E. Mosher , M. Futai , R.H. Fillingame
DOI: 10.1016/S0021-9258(19)70240-3
关键词:
摘要: The proton-translocating ATPase complex (F1F0) of Escherichia coli was purified after inductin a lambda-transducing phage (lambda asn5) carrying the genes th unc operon. activity membranes prepared from induced lambda-unc lysogen 6-fold greater than strains lacking unc-transducing phage, confirming report Kanazawa et al. (1979) Proc. Natl. Acad. Sci. U. S. A. 76, 1126-1130). F1F0-ATPase in comparable yield either enriched or control using modification procedure reported by Foster and Fillingame ((1979) J. Biol. Chem. 254, 8230-8236). EAch eight subunits that had been as components F1F0 wild type E. overproduced lysogen. All co-purified same stoichiometric proportion coli. We conclude all are likely coded small segment chromosomal DNA carried phage. These experiments provide first evidence polypeptides authentic rather contaminants fortuitously co-purify.
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