Soluble and Membrane ATPases of Mitochondria, Chloroplasts, and Bacteria: Molecular Structure, Enzymatic Properties, and Functions

摘要: Publisher Summary This chapter discusses the molecular structure, enzymatic properties, and functions of soluble membrane ATPases mitochondria, chloroplasts, bacteria. In intact organelle (or system in bacteria), electrontransport process ATP production are tightly coupled. Oxidation does not seem to occur unless energy is utilized purposefully. There evidence that energized state can also be coupled directly processes such as transport. Once formed, it broken down for a specific function. Thus, high ATPase activity fractions derived from mitochondria clearly artifacts. They useful artifacts designing experiments understand some aspects mechanism action enzyme system. The information obtained by examining binding, regulation binding identification subunits concerned with their regulation, bound immense value. Progress on this aspect may fast since standard methods enzymology protein chemistry applied readily. However, hydrolytic step or little value, and, fact, hindrance, understanding coupling oxidative synthesis. occurs hydrophobic environment where site seen F 1 even exist, if exists, accessible water well have quite different conformation.