Oligodendroglioma cells shed microvesicles which contain TRAIL as well as molecular chaperones and induce cell death in astrocytes.

摘要: Microvesicles (MVs) shed from G26/24 oligodendroglioma cells were previously reported to cause a reproducible, dose-dependent, inhibitory effect on neurite outgrowth, and eventually neuronal apoptosis, when added primary cultures of rat cortical neurons. These effects reduced but not abolished by functional monoclonal antibodies against Fas-L. In order investigate whether MVs contain other factors able induce cell death, we tested them for TRAIL found clear evidence its presence in the vesicles. This finding suggests possibility that Fas-L cooperate inducing brain death. Aimed at understanding route through which vesicles deliver their messages target cells, labeled with radioactive methionine then tumor unlabeled astrocytes culture. Here report proteins delivered test cells. astrocytes, like neurons, are sensitive oligodendroglioma-derived vesicles, prepared media conditioned astrocytes. clearly more resistant than neurons microvesicle-induced damage: high dose (40 µg) induced death only about 40% Finally, demonstrated Hsp70 is specifically enriched also contain, even if lower level, Hsc70 constitutive chaperone.