Experimental study on the use of a chlorhexidine-loaded carboxymethylcellulose gel as antibacterial coating for hernia repair meshes.

摘要: Biomaterials with an antimicrobial coating could avoid mesh-associated infection following hernia repair. This study assesses the use of a chlorhexidine-loaded carboxymethylcellulose gel in model Staphylococcus aureus mesh infection. A 1% containing 0.05% chlorhexidine was prepared and tested vitro vivo. The tests were antibacterial activity (S. aureus; agar diffusion test) cytotoxicity compared to aqueous (fibroblasts; alamarBlue). For vivo study, partial abdominal wall defects (5 × 2 cm) created New Zealand white rabbits (n = 15) inoculated 0.25 mL S. (106 CFU/mL). Defects repaired lightweight polypropylene (Optilene) without (n = 3) or coated (n = 6) (n = 6). Fourteen days after surgery, bacterial adhesion implant (sonication, immunohistochemistry), host tissue incorporation (light microscopy) macrophage reaction (immunohistochemistry) examined. Carboxymethylcellulose significantly reduced toxicity (p < 0.001) limiting its activity. While control gel-coated implants intensely contaminated, chlorhexidine-gel-coated meshes showed bacteria-free surface, only one specimen signs. this last group (p < 0.05) groups. When incorporated gel, yet maintained bactericidal effect at surgery site. Our findings suggest that for repair prevent surface have no detrimental effects on wound