Urothelium-derived Sonic hedgehog promotes mesenchymal proliferation and induces bladder smooth muscle differentiation

摘要: Induction of smooth muscle differentiation from bladder mesenchyme depends on signals that originate the urothelium. We hypothesize Sonic hedgehog (Shh) is urothelial signal promotes mesenchymal proliferation and induces differentiation. Pregnant FVB mice were euthanized embryonic day (E) 12.5 fetal bladders harvested. Two experimental protocols utilized: Specimens sized by serial sectioning. Cell counts performed after trypsin digestion. Immunohistochemistry was to detect muscle-specific protein expression. alpha-Actin expression quantified using Western blot. All specimens viable at 72h. BLM cultured without Shh survived but did not grow or undergo IB with grew expressed proteins larger contained more cells than (all p<0.05). Increasing concentration 48 480nM change size, cell counts, level alpha-actin Prior culture, express alpha-actin. After culture in Shh-deficient media, detected throughout except submucosal layer. The submucosa thinner 48nM completely obliterated Shh. In mouse bladders, urothelium-derived necessary for affects patterning muscle.