Sonic Hedgehog Functions as a Mitogen During Bell Stage of Odontogenesis

摘要: Epithelial-mesenchymal interactions are required for tissue growth and gene expression patterns during odontogenesis. We showed previously that Sonic hedgehog (SHH) is detectable in both dental epithelium mesenchyme, while Shh transcripts present only, suggesting SHH functions as an autocrine signal a paracrine mesenchyme. This hypothesis was tested here. found by situ hybridization the receptor Ptch-2 indeed expressed whereas Ptc Bovine bell stage tooth germs were microsurgically separated into epithelial mesenchymal portions resulting fragments organ-cultured. In cultured themselves, of Gli-1 (a putative transcriptional mediator signaling) significantly decreased inner stratum intermedium layers; this accompanied sharp ...